Issue 19, 2017

Gold nanoparticle aggregation enables colorimetric sensing assays for enzymatic decarboxylation

Abstract

Monitoring enzymatic processes is of fundamental importance for the understanding of the associated biological phenomena. In this work, we show that amino acid decarboxylase activity can be readily monitored by a colorimetric assay with gold nanoparticles (AuNPs), which undergo selective product-induced aggregation. Once the carboxylate group is eliminated, the resulting diamine products trigger AuNP aggregation, leading to a significant color change. Furthermore, this new method provides a rapid way for potential inhibitor screening.

Graphical abstract: Gold nanoparticle aggregation enables colorimetric sensing assays for enzymatic decarboxylation

Supplementary files

Article information

Article type
Communication
Submitted
09 Mar 2017
Accepted
18 Apr 2017
First published
19 Apr 2017

Anal. Methods, 2017,9, 2784-2787

Gold nanoparticle aggregation enables colorimetric sensing assays for enzymatic decarboxylation

M. Nilam, A. Hennig, W. M. Nau and K. I. Assaf, Anal. Methods, 2017, 9, 2784 DOI: 10.1039/C7AY00642J

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