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Issue 12, 2017
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Quantum dot-based sensitive detection of disease specific exosome in serum

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Abstract

Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related. Here, we used CdSe quantum dot (CdSeQD) functionalised-biotinylated HER-2 and FAM134B antibodies as breast and colon cancer markers. After magnetic washing and purification steps, acid dissolution of CdSeQDs and subsequent anodic stripping voltammetric quantification of Cd2+ were carried out at the bare glassy carbon working electrode. This method enabled sensitive detection of 100 exosomes per μL with a relative standard deviation (%RSD) of <5.5% in cancer cell lines and a small cohort of serum samples (n = 9) collected from patients with colorectal adenocarcinoma. We believe that our approach could potentially represent an effective bioassay for the quantification of disease-specific exosomes in clinical samples.

Graphical abstract: Quantum dot-based sensitive detection of disease specific exosome in serum

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Supplementary files

Article information


Submitted
23 Apr 2017
Accepted
02 May 2017
First published
04 May 2017

Analyst, 2017,142, 2211-2219
Article type
Paper

Quantum dot-based sensitive detection of disease specific exosome in serum

K. Boriachek, Md. N. Islam, V. Gopalan, A. K. Lam, N. Nguyen and M. J. A. Shiddiky, Analyst, 2017, 142, 2211
DOI: 10.1039/C7AN00672A

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