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Issue 19, 2017
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A multiplex microplatform for the detection of multiple DNA methylation events using gold–DNA affinity

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Abstract

We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of a micro-fabricated multiplex device comprising a microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg μl−1 of DNA with no sequencing requirement.

Graphical abstract: A multiplex microplatform for the detection of multiple DNA methylation events using gold–DNA affinity

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Supplementary files

Article information


Submitted
11 Apr 2017
Accepted
15 Aug 2017
First published
16 Aug 2017

Analyst, 2017,142, 3573-3578
Article type
Communication

A multiplex microplatform for the detection of multiple DNA methylation events using gold–DNA affinity

A. A. I. Sina, M. T. Foster, D. Korbie, L. G. Carrascosa, M. J. A. Shiddiky, J. Gao, S. Dey and M. Trau, Analyst, 2017, 142, 3573
DOI: 10.1039/C7AN00611J

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