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Issue 8, 2017
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Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

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Abstract

Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling strategies is set to revolutionise the direct visualisation of intracellular drug uptake. Rational evaluation of a series of Raman-active labels has allowed the identification of highly active labels which have minimal perturbation on the biological efficacy of the parent drug. Drug uptake has been correlated with markers of cellular composition and cell cycle status, and mapped across intracellular structures using dual-colour and multi-modal imaging. The minimal phototoxicity and low photobleaching associated with SRS microscopy has enabled real-time imaging in live cells. These studies demonstrate the potential for SRS microscopy in the drug development process.

Graphical abstract: Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

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Supplementary files

Article information


Submitted
25 Apr 2017
Accepted
15 May 2017
First published
24 May 2017

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2017,8, 5606-5615
Article type
Edge Article

Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

W. J. Tipping, M. Lee, A. Serrels, V. G. Brunton and A. N. Hulme, Chem. Sci., 2017, 8, 5606
DOI: 10.1039/C7SC01837A

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