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Issue 81, 2017, Issue in Progress
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Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles

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Abstract

The styrene–maleic acid (SMA) copolymers enable a detergent-free extraction of membrane proteins from lipid bilayers yielding stable water-soluble nanocontainers with lipid-encased proteins. Here, we demonstrate the ability of the SMA copolymer to extract a complex of interacting membrane proteins from proteoliposomes, namely the archaeal sensory rhodopsin II from Natronomonas pharaonis, NpSRII, in complex with its transducer, NpHtrII. The NpSRII/NpHtrII complexes retained their structural integrity within SMA lipid particles as revealed by diverse biophysical techniques. Pulse electron paramagnetic resonance data showed the SMA-encased NpSRII/NpHtrII complexes as native-like 2 : 2 dimers with a distance between NpSRII sites 159 according to the “V”-shaped conformation found in a crystal structure.

Graphical abstract: Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles

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Publication details

The article was received on 28 Sep 2017, accepted on 26 Oct 2017 and first published on 03 Nov 2017


Article type: Paper
DOI: 10.1039/C7RA10756K
RSC Adv., 2017,7, 51324-51334
  • Open access: Creative Commons BY license
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    Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles

    N. Voskoboynikova, W. Mosslehy, A. Colbasevici, T. T. Ismagulova, D. V. Bagrov, A. A. Akovantseva, P. S. Timashev, A. Y. Mulkidjanian, V. N. Bagratashvili, K. V. Shaitan, M. P. Kirpichnikov and H.-J. Steinhoff, RSC Adv., 2017, 7, 51324
    DOI: 10.1039/C7RA10756K

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