Jump to main content
Jump to site search

Issue 67, 2017
Previous Article Next Article

Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model

Author affiliations

Abstract

Because of the limitations of current scaffolds and unfavorable results of clinical trials, proper scaffolds facilitating bladder reconstruction are highly desirable. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional bladder acellular matrix graft (BAMG) in a rat model of bladder augmentation. Twenty-four 8 week-old male Sprague-Dawley rats were randomly assigned to the chitosan scaffold, BAMG and cystotomy groups. The rats' bladders were sampled for cystography and routine histological examination at 21 and 70 days. Immunofluorescence photometry, conscious cystometry, quantitative real-time polymerase chain reaction, and western blot analyses were performed using bladders at 70 days. Compared with BAMG, the chitosan scaffold consisted of a membrane-like compact layer and a sponge-like porous layer with an excellent combination of mechanical strength and flexibility. The chitosan group showed better performances than the BAMG group in radiographic cystography, smooth muscle regeneration, blood vessel numbers and functional restoration. In contrast to reduced bladder compliance induced by BAMG, bladder augmented by chitosan displayed nearly 1.5-fold increased bladder capacity with comparable compliance to that of the cystotomy group at 70 days. The chitosan group exhibited higher levels of VEGF and VEGFR2, associated with the activation of the hypoxia-related SDF-1α/CXCR4 pathway. These results suggested that the asymmetric bilayer chitosan scaffold is a promising scaffold for bladder reconstruction.

Graphical abstract: Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Jul 2017, accepted on 24 Aug 2017 and first published on 04 Sep 2017


Article type: Paper
DOI: 10.1039/C7RA07601K
RSC Adv., 2017,7, 42579-42589
  • Open access: Creative Commons BY license
  •   Request permissions

    Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model

    D. Xiao, Q. Wang, H. Yan, A. Qin, X. Lv, Y. Zhao, M. Zhang, Z. Zhou, J. Xu, Q. Hu and M. Lu, RSC Adv., 2017, 7, 42579
    DOI: 10.1039/C7RA07601K

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements