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Issue 35, 2017, Issue in Progress
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Enhanced saccharification of cellulose and sugarcane bagasse by Clostridium thermocellum cultures with Triton X-100 and β-glucosidase/Cellic®CTec2 supplementation

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Abstract

Clostridium thermocellum is the most efficient cellulose-degrading bacterium known for producing cellulosome, which was used to directly degrade sugarcane bagasse (SCB) with limited glucose accumulation. However, the glucose production was significantly enhanced with 0.25% Triton X-100 and 30 U g−1 of β-glucosidase supplementation. A glucose accumulation of 21.33 ± 0.35 g L−1 and substrate degradation of 61.91 ± 0.77% from 60 g L−1 sodium hydroxide-pretreated SCB were reached, with a 609.41% and 75.53% increase over the control (3.01 ± 0.26 g L−1, 35.27 ± 2.91%), respectively. More interestingly, a synergism between C. thermocellum cultures and Cellic®CTec2 (30 CBU per g, 1.2 FPU per g substrate) in the enzymolysis of Avicel was observed and the glucose concentration and saccharification ratio of Avicel reached 56.90 ± 1.20 g L−1 and 94.8% at 60 g L−1 Avicel. Moreover, a highest glucose concentration of 83.17 g L−1 was achieved at 100 g L−1 Avicel. The present work provides an effective and promising process for industrial saccharification of cellulose.

Graphical abstract: Enhanced saccharification of cellulose and sugarcane bagasse by Clostridium thermocellum cultures with Triton X-100 and β-glucosidase/Cellic®CTec2 supplementation

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Publication details

The article was received on 28 Feb 2017, accepted on 04 Apr 2017 and first published on 18 Apr 2017


Article type: Paper
DOI: 10.1039/C7RA02477K
RSC Adv., 2017,7, 21360-21365
  • Open access: Creative Commons BY-NC license
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    Enhanced saccharification of cellulose and sugarcane bagasse by Clostridium thermocellum cultures with Triton X-100 and β-glucosidase/Cellic®CTec2 supplementation

    X. Qu, B. Hu and M. Zhu, RSC Adv., 2017, 7, 21360
    DOI: 10.1039/C7RA02477K

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