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Issue 38, 2017
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Preliminary characterization and immunostimulatory activity of a novel functional polysaccharide from Astragalus residue fermented by Paecilomyces sinensis

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Abstract

An Astragalus residue was reutilized through solid-state fermentation by Paecilomyces sinensis, which is a member of Ophiocordyceps sinensis (Berk.) Sacc. In this research, the polysaccharide from the product of solid-state fermentation primarily was studied. First, HPLC, FT-IR, and GC were applied to study the preliminary characterization of a polysaccharide (APP-II) from the solid-state fermentation product Astragalus residue and Paecilomyces sinensis. Results indicated that APP-II is α-pyran polysaccharide with an average molecular weight of 670 kD that is composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 4.0 : 4.2 : 2.0 : 41.4 : 22.5 : 25.9. Second, an immunostimulatory activity of APP-I and APP-II was detected. Results suggested that APP-I and APP-II could promote RAW264.7 cell proliferation. APP-I was used to detect the immunostimulatory activity of APP-II in vivo. The results indicated that APP-I can promote growth of immune organs, stimulate splenocyte proliferation, and enhance NK activity; this indicated that APP-II had immunostimulatory activity in vivo. All the results indicated that APP-II is a novel polysaccharide with immunostimulatory activity.

Graphical abstract: Preliminary characterization and immunostimulatory activity of a novel functional polysaccharide from Astragalus residue fermented by Paecilomyces sinensis

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Article information


Submitted
30 Jan 2017
Accepted
20 Apr 2017
First published
03 May 2017

This article is Open Access

RSC Adv., 2017,7, 23875-23881
Article type
Paper

Preliminary characterization and immunostimulatory activity of a novel functional polysaccharide from Astragalus residue fermented by Paecilomyces sinensis

H. Sun, Z. Zhu, X. Yang, M. Meng, L. Dai and Y. Zhang, RSC Adv., 2017, 7, 23875
DOI: 10.1039/C7RA01279A

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