Issue 38, 2017

Preliminary characterization and immunostimulatory activity of a novel functional polysaccharide from Astragalus residue fermented by Paecilomyces sinensis

Abstract

An Astragalus residue was reutilized through solid-state fermentation by Paecilomyces sinensis, which is a member of Ophiocordyceps sinensis (Berk.) Sacc. In this research, the polysaccharide from the product of solid-state fermentation primarily was studied. First, HPLC, FT-IR, and GC were applied to study the preliminary characterization of a polysaccharide (APP-II) from the solid-state fermentation product Astragalus residue and Paecilomyces sinensis. Results indicated that APP-II is α-pyran polysaccharide with an average molecular weight of 670 kD that is composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 4.0 : 4.2 : 2.0 : 41.4 : 22.5 : 25.9. Second, an immunostimulatory activity of APP-I and APP-II was detected. Results suggested that APP-I and APP-II could promote RAW264.7 cell proliferation. APP-I was used to detect the immunostimulatory activity of APP-II in vivo. The results indicated that APP-I can promote growth of immune organs, stimulate splenocyte proliferation, and enhance NK activity; this indicated that APP-II had immunostimulatory activity in vivo. All the results indicated that APP-II is a novel polysaccharide with immunostimulatory activity.

Graphical abstract: Preliminary characterization and immunostimulatory activity of a novel functional polysaccharide from Astragalus residue fermented by Paecilomyces sinensis

Article information

Article type
Paper
Submitted
30 Jan 2017
Accepted
20 Apr 2017
First published
03 May 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 23875-23881

Preliminary characterization and immunostimulatory activity of a novel functional polysaccharide from Astragalus residue fermented by Paecilomyces sinensis

H. Sun, Z. Zhu, X. Yang, M. Meng, L. Dai and Y. Zhang, RSC Adv., 2017, 7, 23875 DOI: 10.1039/C7RA01279A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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