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Issue 28, 2017, Issue in Progress
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Hierarchically templated beads with tailored pore structure for phosphopeptide capture and phosphoproteomics

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Abstract

Two templating approaches to produce imprinted phosphotyrosine capture beads with a controllable pore structure are reported and compared with respect to their ability to enrich phosphopeptides from a tryptic peptide mixture. The beads were prepared by the polymerization of urea-based host monomers and crosslinkers inside the pores of macroporous silica beads with both free and immobilized template. In the final step the silica was removed by fluoride etching resulting in mesoporous polymer replicas with narrow pore size distributions, pore diameters ≈ 10 nm and surface area > 260 m2 g−1. The beads displayed pronounced phosphotyrosine affinity and selectivity in binding tests using model peptides in acetonitrile rich solutions with a performance surpassing solution polymerized bulk imprinted materials. Tests of the beads for the enrichment of phosphopeptides from tryptic digests of twelve proteins revealed both pY/pS and pY/Y selectivity. This was reflected in a nearly 6-fold increase in the enrichment factor of a 23-mer pY-peptide and pY/pS normalized intensity ratios up to 1.5, when comparing the template mesoporous beads with the bulk materials.

Graphical abstract: Hierarchically templated beads with tailored pore structure for phosphopeptide capture and phosphoproteomics

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Supplementary files

Article information


Submitted
10 Jan 2017
Accepted
12 Mar 2017
First published
17 Mar 2017

This article is Open Access

RSC Adv., 2017,7, 17154-17163
Article type
Paper

Hierarchically templated beads with tailored pore structure for phosphopeptide capture and phosphoproteomics

C. Wierzbicka, S. B. Torsetnes, O. N. Jensen, S. Shinde and B. Sellergren, RSC Adv., 2017, 7, 17154
DOI: 10.1039/C7RA00385D

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