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Issue 32, 2017
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Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection

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Abstract

In this report, we show how a convenient on-resin copper-click functionalization of azido-functionalized peptide nucleic acids (PNAs) allows various PNA-based detection strategies. Firstly, a thiazole orange (TO) clicked PNA probe facilitates a binary readout when combined with F/Q labeled DNA, giving increased sensitivity for antisense detection. Secondly, our TO-PNA conjugate also allows single nucleotide polymorphism detection. Since antisense detection is also possible in the absence of the TO label, our sensing platform based on azido-D-ornithine containing PNA even allows for additional and more advanced functionalization and sensing strategies.

Graphical abstract: Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection

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Supplementary files

Article information


Submitted
30 Jun 2017
Accepted
25 Jul 2017
First published
25 Jul 2017

Org. Biomol. Chem., 2017,15, 6710-6714
Article type
Communication

Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection

D. Gahtory, M. Murtola, M. M. J. Smulders, T. Wennekes, H. Zuilhof, R. Strömberg and B. Albada, Org. Biomol. Chem., 2017, 15, 6710
DOI: 10.1039/C7OB01592E

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