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Issue 5, 2017
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Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens

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Abstract

Rapid and sensitive point-of-care diagnostics are of paramount importance for early detection of infectious diseases and timely initiation of treatment. Here, we present cellulose paper and flexible plastic chips with printed graphene-modified silver electrodes as universal point-of-care diagnostic tools for the rapid and sensitive detection of microbial pathogens or nucleic acids through utilizing electrical sensing modality and loop-mediated isothermal amplification (LAMP). We evaluated the ability of the developed paper-based assay to detect (i) viruses on cellulose-based paper microchips without implementing amplification in samples with viral loads between 106 and 108 copies per ml, and (ii) amplified HIV-1 nucleic acids in samples with viral loads between 10 fg μl−1 and 108 fg μl−1. The target HIV-1 nucleic acid was amplified using the RT-LAMP technique and detected through the electrical sensing of LAMP amplicons for a broad range of RNA concentrations between 10 fg μl−1 and 108 fg μl−1 after 40 min of amplification time. Our assay may be used for antiretroviral therapy monitoring where it meets the sensitivity requirement of the World Health Organization guidelines. Such a paper microchip assay without the amplification step may also be considered as a simple and inexpensive approach for acute HIV detection where maximum viral replication occurs.

Graphical abstract: Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens

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Supplementary files

Article information


Submitted
13 Aug 2016
Accepted
27 Oct 2016
First published
28 Oct 2016

Nanoscale, 2017,9, 1852-1861
Article type
Paper

Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens

M. Safavieh, V. Kaul, S. Khetani, A. Singh, K. Dhingra, M. K. Kanakasabapathy, M. S. Draz, A. Memic, D. R. Kuritzkes and H. Shafiee, Nanoscale, 2017, 9, 1852
DOI: 10.1039/C6NR06417E

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