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Issue 10, 2017
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Real-time in situ analysis of biocorona formation and evolution on silica nanoparticles in defined and complex biological environments

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Abstract

Biomolecules such as proteins immediately adsorb on the surface of nanoparticles upon their exposure to a biological environment. The formed adlayer is commonly referred to as biomolecule corona (biocorona) and defines the biological activity and toxicity of the nanoparticle. Therefore, it is essential to understand in detail the biocorona formation process, and how it is governed by parameters like composition of the biological environment, and nanoparticle size, shape and faceting. Here we present a detailed equilibrium and real time in situ study of biocorona formation at SiO2-nanoparticle surfaces upon exposure to defined (BSA, IgG) and complex (bovine serum, IgG depleted bovine serum) biological samples. We use both nanofabricated surface-associated Au core–SiO2 shell nanoparticles (faceted, d = 92–167 nm) with integrated nanoplasmonic sensing function and dispersed SiO2 nanoparticles (using DLS and SDS-PAGE). The results show that preadsorbed BSA or IgG are exchanged for other proteins when exposed to bovine serum. In addition, the results show that IgG forms a biocorona with different properties at curved (edge) and flat (facet) SiO2-nanoparticle surfaces. Our study paves the way for further real time in situ investigations of the biocorona formation and evolution kinetics, as well as the role of molecular orientation in biocorona formation, on nanoparticles with surface faceting.

Graphical abstract: Real-time in situ analysis of biocorona formation and evolution on silica nanoparticles in defined and complex biological environments

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Supplementary files

Article information


Submitted
12 Aug 2016
Accepted
23 Feb 2017
First published
23 Feb 2017

This article is Open Access

Nanoscale, 2017,9, 3620-3628
Article type
Paper

Real-time in situ analysis of biocorona formation and evolution on silica nanoparticles in defined and complex biological environments

R. Frost, C. Langhammer and T. Cedervall, Nanoscale, 2017, 9, 3620
DOI: 10.1039/C6NR06399C

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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