A simple and facile NMR method for the determination of hydrogen bonding by amide N–H protons in protein models and other compounds

Abstract

It is shown that measurements of the ¹H chemical shifts of amide N–H protons in chloroform and in DMSO solvents are sufficient to determine the extent of hydrogen bonding of the N–H protons in a variety of compounds containing the amide group. Two recent examples are presented from protein and structural chemistry.