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Issue 2, 2017
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Transcriptome profile of yeast reveals the essential role of PMA2 and uncharacterized gene YBR056W-A (MNC1) in adaptation to toxic manganese concentration

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Abstract

Adaptation of S. cerevisiae to toxic concentrations of manganese provides a physiological model of heavy metal homeostasis. Transcriptome analysis of adapted yeast cells reveals upregulation of cell wall and plasma membrane proteins including membrane transporters. The gene expression in adapted cells differs from that of cells under short-term toxic metal stress. Among the most significantly upregulated genes are PMA2, encoding an ortholog of Pma1 H+-ATPase of the plasma membrane, and YBR056W-A, encoding a putative membrane protein Mnc1 that belongs to the CYSTM family and presumably chelates manganese at the cell surface. We demonstrate that these genes are essential for the adaptation to toxic manganese concentration and propose an extended scheme of manganese detoxification in yeast.

Graphical abstract: Transcriptome profile of yeast reveals the essential role of PMA2 and uncharacterized gene YBR056W-A (MNC1) in adaptation to toxic manganese concentration

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Article information


Submitted
23 Sep 2016
Accepted
09 Jan 2017
First published
09 Jan 2017

This article is Open Access

Metallomics, 2017,9, 175-182
Article type
Paper

Transcriptome profile of yeast reveals the essential role of PMA2 and uncharacterized gene YBR056W-A (MNC1) in adaptation to toxic manganese concentration

N. Andreeva, E. Kulakovskaya, A. Zvonarev, A. Penin, I. Eliseeva, A. Teterina, A. Lando, I. V. Kulakovskiy and T. Kulakovskaya, Metallomics, 2017, 9, 175
DOI: 10.1039/C6MT00210B

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