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Issue 1, 2017
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Proteomics profiling of interactome dynamics by colocalisation analysis (COLA)

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Abstract

Localisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation of two proteins can therefore be indicative of their functional association. We here present COLA, a proteomics based strategy coupled with a bioinformatics framework to detect protein–protein co-localisations on a global scale. COLA reveals functional interactions by matching proteins with significant similarity in their subcellular localisation signatures. The rapid nature of COLA allows mapping of interactome dynamics across different conditions or treatments with high precision.

Graphical abstract: Proteomics profiling of interactome dynamics by colocalisation analysis (COLA)

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Supplementary files

Article information


Submitted
12 Oct 2016
Accepted
01 Nov 2016
First published
01 Nov 2016

This article is Open Access

Mol. BioSyst., 2017,13, 92-105
Article type
Paper

Proteomics profiling of interactome dynamics by colocalisation analysis (COLA)

F. K. Mardakheh, H. Z. Sailem, S. Kümper, C. J. Tape, R. R. McCully, A. Paul, S. Anjomani-Virmouni, C. Jørgensen, G. Poulogiannis, C. J. Marshall and C. Bakal, Mol. BioSyst., 2017, 13, 92
DOI: 10.1039/C6MB00701E

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