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Issue 16, 2017

Spectral reading of optical resonance-encoded cells in microfluidics

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Abstract

The ability to label individual cells is useful for single-cell-level studies of complex cellular interactions and heterogeneity. Optically readable cell labeling is attractive as it can be investigated non-invasively and repeatedly at high speeds. Here, we demonstrate the feasibility of large-scale cell barcoding and identification using fluorescent polystyrene microbeads loaded into cells. Intracellular beads with different diameters in a range of 5 to 12 μm generate spectrally distinguished features or barcodes. A microfluidic chip was used to measure fluorescence resonance peaks emitted from individual cells. An algorithm comparing the peak wavelengths to a reference barcode library allowed barcode identification with high accuracy. This work provides a guideline to increase the number of unique identifiers and reduce various false-positive and false-negative errors.

Graphical abstract: Spectral reading of optical resonance-encoded cells in microfluidics

Supplementary files

Article information


Submitted
02 Mar 2017
Accepted
28 Jun 2017
First published
07 Jul 2017

Lab Chip, 2017,17, 2777-2784
Article type
Paper

Spectral reading of optical resonance-encoded cells in microfluidics

M. Humar, A. Upadhya and S. H. Yun, Lab Chip, 2017, 17, 2777 DOI: 10.1039/C7LC00220C

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