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Issue 7, 2017
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A fully disposable and integrated paper-based device for nucleic acid extraction, amplification and detection

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Abstract

Nucleic acid testing (NAT) has been widely used for disease diagnosis, food safety control and environmental monitoring. At present, NAT mainly involves nucleic acid extraction, amplification and detection steps that heavily rely on large equipment and skilled workers, making the test expensive, time-consuming, and thus less suitable for point-of-care (POC) applications. With advances in paper-based microfluidic technologies, various integrated paper-based devices have recently been developed for NAT, which however require off-chip reagent storage, complex operation steps and equipment-dependent nucleic acid amplification, restricting their use for POC testing. To overcome these challenges, we demonstrate a fully disposable and integrated paper-based sample-in-answer-out device for NAT by integrating nucleic acid extraction, helicase-dependent isothermal amplification and lateral flow assay detection into one paper device. This simple device allows on-chip dried reagent storage and equipment-free nucleic acid amplification with simple operation steps, which could be performed by untrained users in remote settings. The proposed device consists of a sponge-based reservoir and a paper-based valve for nucleic acid extraction, an integrated battery, a PTC ultrathin heater, temperature control switch and on-chip dried enzyme mix storage for isothermal amplification, and a lateral flow test strip for naked-eye detection. It can sensitively detect Salmonella typhimurium, as a model target, with a detection limit of as low as 102 CFU ml−1 in wastewater and egg, and 103 CFU ml−1 in milk and juice in about an hour. This fully disposable and integrated paper-based device has great potential for future POC applications in resource-limited settings.

Graphical abstract: A fully disposable and integrated paper-based device for nucleic acid extraction, amplification and detection

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Supplementary files

Article information


Submitted
27 Dec 2016
Accepted
02 Mar 2017
First published
08 Mar 2017

Lab Chip, 2017,17, 1270-1279
Article type
Paper

A fully disposable and integrated paper-based device for nucleic acid extraction, amplification and detection

R. Tang, H. Yang, Y. Gong, M. You, Z. Liu, J. R. Choi, T. Wen, Z. Qu, Q. Mei and F. Xu, Lab Chip, 2017, 17, 1270
DOI: 10.1039/C6LC01586G

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