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Issue 3, 2017
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3D microvascular model recapitulates the diffuse large B-cell lymphoma tumor microenvironment in vitro

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Abstract

Diffuse large B-cell lymphoma (DLBCL) is an aggressive cancer that affects ∼22 000 people in the United States yearly. Understanding the complex cellular interactions of the tumor microenvironment is critical to the success and development of DLBCL treatment strategies. In vitro platforms that successfully model the complex tumor microenvironment without introducing the variability of in vivo systems are vital for understanding these interactions. To date, no such in vitro model exists that can accurately recapitulate the interactions that occur between immune cells, cancer cells, and endothelial cells in the tumor microenvironment of DLBCL. To that end, we developed a lymphoma-on-chip model consisting of a hydrogel based tumor model traversed by a vascularized, perfusable, round microchannel that successfully recapitulates key complexities and interactions of the in vivo tumor microenvironment in vitro. We have shown that the perfusion capabilities of this technique allow us to study targeted treatment strategies, as well as to model the diffusion of infused reagents spatiotemporally. Furthermore, this model employs a novel fabrication technique that utilizes common laboratory materials, and allows for the microfabrication of multiplex microvascular environments without the need for advanced microfabrication facilities. Through our facile microfabrication process, we are able to achieve micro vessels within a tumor model that are highly reliable and precise over the length of the vessel. Overall, we have developed a tool that enables researchers from many diverse disciplines to study previously inaccessible aspects of the DLBCL tumor microenvironment, with profound implications for drug delivery and design.

Graphical abstract: 3D microvascular model recapitulates the diffuse large B-cell lymphoma tumor microenvironment in vitro

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Supplementary files

Article information


Submitted
24 Sep 2016
Accepted
23 Dec 2016
First published
23 Dec 2016

Lab Chip, 2017,17, 407-414
Article type
Technical Innovation

3D microvascular model recapitulates the diffuse large B-cell lymphoma tumor microenvironment in vitro

R. G. Mannino, A. N. Santiago-Miranda, P. Pradhan, Y. Qiu, J. C. Mejias, S. S. Neelapu, K. Roy and W. A. Lam, Lab Chip, 2017, 17, 407
DOI: 10.1039/C6LC01204C

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