Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 10, 2017
Previous Article Next Article

In vitro and in vivo inhibitory effects of a Pleurotus eryngii protein on colon cancer cells

Author affiliations

Abstract

Edible mushrooms have been considered as a good source of bioactive components with various health benefits. Our previous study demonstrated the identity of a novel protein from Pleurotus eryngii, namely PEP, and its anti-inflammatory activities. Herein, we further determined the inhibitory effects of PEP against colon cancer cells. PEP suppressed the proliferation of human and murine colon cancer HCT116 and MC38 cells in a dose and time-dependent fashion, while it showed no inhibitory effect on normal human colonic myofibroblasts CCD-18Co at the same concentrations tested. Moreover, PEP induced cell cycle arrest and led to extensive cellular apoptosis in colon cancer cells, which was associated with the downregulation of cell cycle-related signaling proteins, e.g. cyclin B, cyclin E and cdc-2, and the upregulation of apoptosis-related signaling proteins, e.g. p53 and c-PARP. The results from an in vivo study showed that PEP treatment significantly suppressed tumor development of allograft colon cancer cells in mice, and this inhibition was associated with the upregulation of p21, p53, caspase-3 and cleaved caspase-3. Overall, our results provided a basis for PEP as a promising preventive agent against colon cancer.

Graphical abstract: In vitro and in vivo inhibitory effects of a Pleurotus eryngii protein on colon cancer cells

Back to tab navigation

Article information


Submitted
17 Jun 2017
Accepted
14 Aug 2017
First published
18 Aug 2017

Food Funct., 2017,8, 3553-3562
Article type
Paper

In vitro and in vivo inhibitory effects of a Pleurotus eryngii protein on colon cancer cells

B. Yuan, N. Ma, L. Zhao, E. Zhao, Z. Gao, W. Wang, M. Song, G. Zhang, Q. Hu and H. Xiao, Food Funct., 2017, 8, 3553
DOI: 10.1039/C7FO00895C

Social activity

Search articles by author

Spotlight

Advertisements