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Issue 96, 2017
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Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

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Abstract

We found that Escherichia coli MazF toxin, an ACA-sequence-specific endoribonuclease, was sensitive to N6-methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Furthermore, the approach could be used for inhibitor screening.

Graphical abstract: Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

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Article information


Submitted
04 Oct 2017
Accepted
12 Nov 2017
First published
13 Nov 2017

Chem. Commun., 2017,53, 12930-12933
Article type
Communication

Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

M. Imanishi, S. Tsuji, A. Suda and S. Futaki, Chem. Commun., 2017, 53, 12930
DOI: 10.1039/C7CC07699A

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