Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5

Thirupathi Ravula; Carlo Barnaba; Mukesh Mahajan; G. M. Anantharamaiah; Sang-Choul Im; Lucy Waskell; Ayyalusamy Ramamoorthy

doi:10.1039/C7CC07520K

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b₅†

Thirupathi Ravula,^a Carlo Barnaba,^a Mukesh Mahajan,

^a G. M. Anantharamaiah,^b Sang-Choul Im,^c Lucy Waskell^c and Ayyalusamy Ramamoorthy

*^a

Author affiliations

* Corresponding authors

^a Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA
E-mail: ramamoor@umich.edu

^b Department of Medicine, UAB Medical Center, Birmingham, Alabama 35294, USA

^c Department of Anesthesiology, University of Michigan and VA Medical Center, Ann Arbor, MI 48105-1055, USA

Abstract

Heme's spin-multiplicity is key in determining the enzymatic function of cytochrome P450 (cytP450). The origin of the low-spin state in ferric P450 is still under debate. Here, we report the first experimental demonstration of P450's membrane interaction altering its spin equilibrium which is accompanied by a stronger affinity for cytochrome b₅. These results highlight the importance of lipid membrane for the function of P450.

Download options Please wait...

Supplementary files

Supplementary information PDF (3655K)

Article information

DOI: https://doi.org/10.1039/C7CC07520K
Article type: Communication
Submitted: 26 Sep 2017
Accepted: 10 Nov 2017
First published: 10 Nov 2017

Download Citation

Chem. Commun., 2017,53, 12798-12801

Permissions

Request permissions

Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b₅

T. Ravula, C. Barnaba, M. Mahajan, G. M. Anantharamaiah, S. Im, L. Waskell and A. Ramamoorthy, Chem. Commun., 2017, 53, 12798 DOI: 10.1039/C7CC07520K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications