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Issue 70, 2017
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Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes

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Abstract

An on-surface isothermal helicase-dependent amplification is devised for simple, point-of-need quantification of bacterial genomes. The method relies on the enzyme-extension of a thiol-modified reverse primer anchored to indium tin oxide electrodes, which shows strikingly high thermal and storage stability. Amplification and electrochemical detection of only 10 genomes are thus performed on the same platform without thermal cycling.

Graphical abstract: Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes

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Article information


Submitted
03 Jul 2017
Accepted
25 Jul 2017
First published
26 Jul 2017

Chem. Commun., 2017,53, 9721-9724
Article type
Communication

Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes

S. Barreda-García, R. Miranda-Castro, N. de-los-Santos-Álvarez, A. J. Miranda-Ordieres and M. J. Lobo-Castañón, Chem. Commun., 2017, 53, 9721
DOI: 10.1039/C7CC05128J

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