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Issue 19, 2017
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Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

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Abstract

We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.

Graphical abstract: Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

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Supplementary files

Article information


Submitted
14 Dec 2016
Accepted
13 Feb 2017
First published
13 Feb 2017

Chem. Commun., 2017,53, 2874-2877
Article type
Communication

Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

H. Soleimaninejad, M. Z. Chen, X. Lou, T. A. Smith and Y. Hong, Chem. Commun., 2017, 53, 2874
DOI: 10.1039/C6CC09916E

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