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Issue 5, 2017
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A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy

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Abstract

Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.

Graphical abstract: A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy

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Article information


Submitted
14 Nov 2016
Accepted
22 Dec 2016
First published
22 Dec 2016

Chem. Commun., 2017,53, 979-982
Article type
Communication

A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy

G. Matela, P. Gao, G. Guigas, A. F. Eckert, K. Nienhaus and G. Ulrich Nienhaus, Chem. Commun., 2017, 53, 979
DOI: 10.1039/C6CC09081H

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