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Issue 13, 2017

New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration

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Abstract

New approaches for fabrication of centrifugal microfluidic platforms (μCDs) for sample micro-extraction and pre-concentration in bioanalytical and environmental applications are presented. The integration of both octadecylsilica (C18) micro-particulate and porous carbon monolithic stationary phases was demonstrated and on-disc extractions of analytes in samples of different nature were performed. A novel strategy based on the packing of micro-particulate stationary phases using porous organic polymer monoliths as column frits was demonstrated through the in situ photo-polymerisation of monolithic frits in a specific area of the micro-channel, thereby greatly facilitating stationary phase packing within μCD platforms. An enrichment factor of 3.7 was obtained for vitamin B12 following on-disc pre-concentration on the octadecylsilica columns. UV-Vis absorbance measurements were also performed in the outlet reservoir permitting quasi-on-line analysis of the small volume fractions collected after extraction, with limits of detection (LODs) found for vitamin B12 (LOD = 43 μM) being rather similar to those found with a commercially available spectrophotometer (LOD = 37 μM). Furthermore, the first integration of carbon monoliths within microfluidic channels is reported. Carbon monoliths were fabricated as rods and cut into discs for their integration within the microfluidic network, offering a highly porous bimodal structure with low flow-through back-pressures, excellent chemical stability as well as adequate mechanical stability. The carbon monolith-based μCD platform was evaluated as a rapid semi-automated pre-concentration approach suitable for in-field use prior to in-lab HPLC quantitation of pollutants at low concentration levels. Calculated mean recoveries for phenol from tap water spiked-samples by using this on-disc pre-concentration method were 68 ± 4% (n = 4, RSD = 5%).

Graphical abstract: New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration

Supplementary files

Article information


Submitted
13 Jan 2017
Accepted
30 Jan 2017
First published
06 Feb 2017

Anal. Methods, 2017,9, 1998-2006
Article type
Paper

New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration

E. Duffy, R. Padovani, X. He, R. Gorkin, E. Vereshchagina, J. Ducrée, E. Nesterenko, P. N. Nesterenko, D. Brabazon, B. Paull and M. Vázquez, Anal. Methods, 2017, 9, 1998 DOI: 10.1039/C7AY00127D

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