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Issue 24, 2017
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Bulk double emulsification for flow cytometric analysis of microfluidic droplets

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Abstract

Droplet microfluidics is valuable for applications in chemistry and biology, but generates massive numbers of droplets that must be analyzed and sorted. Here, we describe a simple approach to bulk double emulsify microfluidic emulsions for analysis and sorting with commercial flow cytometers. We illustrate the method by using it to identify droplets based on nucleic acid content. Though simple, our method provides a general approach for analyzing and sorting microfluidic droplets without custom microfluidic double emulsifiers or sorters.

Graphical abstract: Bulk double emulsification for flow cytometric analysis of microfluidic droplets

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Article information


Submitted
13 Oct 2017
Accepted
16 Oct 2017
First published
13 Nov 2017

This article is Open Access

Analyst, 2017,142, 4618-4622
Article type
Communication

Bulk double emulsification for flow cytometric analysis of microfluidic droplets

D. J. Sukovich, S. C. Kim, N. Ahmed and A. R. Abate, Analyst, 2017, 142, 4618
DOI: 10.1039/C7AN01695F

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