Jump to main content
Jump to site search

Issue 2, 2017
Previous Article Next Article

Creating BHb-imprinted magnetic nanoparticles with multiple binding sites

Author affiliations

Abstract

A kind of protein imprinted over magnetic Fe3O4@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. Magnetic Fe3O4@Au NPs as carrier materials were modified with 4-mercaptophenylboronic acid (MPBA) and mercaptopropionic acid (MPA) to introduce boronic acids and carboxyl groups. Using Bovine Hemoglobin (BHb) as a template, a polydopamine(PDA)-based molecular imprinted film was fabricated to produce a kind of magnetic molecularly imprinted nanoparticle (MMIP), possessing multiple binding sites with benzene-diol, amino groups, boronic acids and carboxyl groups. The MMIPs exhibited an excellent imprinting effect and adsorption capacity (89.65± 0.38 mg g−1) toward the template protein. The results show that the MMIPs reached saturated adsorption at 0.5 mg mL−1 within 90 min. The synthesized MMIPs are suitable for the removal and enrichment of the template protein in proteomics. The strategy of multiple binding sites paves the way for the preparation of functional nanomaterials in molecular imprinting techniques.

Graphical abstract: Creating BHb-imprinted magnetic nanoparticles with multiple binding sites

Back to tab navigation

Article information


Submitted
21 Sep 2016
Accepted
26 Nov 2016
First published
28 Nov 2016

Analyst, 2017,142, 302-309
Article type
Paper

Creating BHb-imprinted magnetic nanoparticles with multiple binding sites

Y. Li, Y. Chen, L. Huang, B. Lou and G. Chen, Analyst, 2017, 142, 302
DOI: 10.1039/C6AN02121B

Social activity

Search articles by author

Spotlight

Advertisements