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Issue 40, 2016
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Clustering and jamming in epithelial–mesenchymal co-cultures

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Abstract

Collective behaviors emerge from coordinated cell–cell interactions during the morphogenesis of tissues and tumors. For instance, cells may display density-dependent phase transitions from a fluid-like “unjammed” phase to a solid-like “jammed” phase, while different cell types can “self-sort”. Here, we comprehensively track single cell dynamics in mixtures of sheet-forming epithelial cells and dispersed mesenchymal cells. We find that proliferating epithelial cells nucleate multicellular clusters that coarsen at a critical density, arresting migration and strengthening spatial velocity correlations. The addition of mesenchymal cells can slow cluster formation and coarsening, resulting in more dispersed individual cells with weak spatial velocity correlations. These behaviors have analogies with a jamming–unjamming transition, where the control parameters are cell density and mesenchymal fraction. This complex interplay of proliferation, clustering and correlated migration may have physical implications for understanding epithelial–mesenchymal interactions in development and disease.

Graphical abstract: Clustering and jamming in epithelial–mesenchymal co-cultures

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Supplementary files

Article information


Submitted
03 Jun 2016
Accepted
12 Sep 2016
First published
12 Sep 2016

Soft Matter, 2016,12, 8327-8337
Article type
Paper

Clustering and jamming in epithelial–mesenchymal co-cultures

M. Gamboa Castro, S. E. Leggett and I. Y. Wong, Soft Matter, 2016, 12, 8327 DOI: 10.1039/C6SM01287F

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