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Issue 8, 2016
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A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues

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Abstract

Herein, we present the rational design, synthesis, characterization, and biological applications of a new rhodamine-based fluorescent probe, HKYellow, for the detection and molecular imaging of peroxynitrite, an important highly reactive oxidant involved in a variety of physiological and pathological processes. HKYellow was rationally designed on the basis of the efficient fluorescence quenching effect of the N-phenyl group to the rhodamine core and a peroxynitrite-triggered N-dearylation reaction to achieve a sensitive and selective fluorescence turn-on response toward peroxynitrite in chemical and biological levels. This probe has been thoroughly evaluated for the robust imaging of peroxynitrite in live cells and tissues. By utilizing HKYellow, we provide the first visual evidence that peroxynitrite is generated in mouse liver tissues under an acute alcohol binge or ischemic–reperfusion condition. This probe should be a powerful molecular imaging tool for interrogating the complex biological roles of peroxynitrite under various biological settings.

Graphical abstract: A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues

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Supplementary files

Article information


Submitted
02 Jan 2016
Accepted
22 Apr 2016
First published
26 Apr 2016

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2016,7, 5407-5413
Article type
Edge Article

A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues

T. Peng, X. Chen, L. Gao, T. Zhang, W. Wang, J. Shen and D. Yang, Chem. Sci., 2016, 7, 5407 DOI: 10.1039/C6SC00012F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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