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Issue 115, 2016, Issue in Progress
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Quantitative analysis of amyloid polymorphism using height histograms to correct for tip convolution effects in atomic force microscopy imaging

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Abstract

Atomic force microscopy (AFM) is widely used to image biological nanostructures. In this work, we demonstrate that AFM can be used to quantitatively characterize the polymorphism of amyloid fibrils through an analysis of height histogram plots under conditions where the image lacks high lateral resolution. In this approach, Gaussian fits are used to determine the characteristic height and volume of fibril species in the height-histogram plot of an image, and to convert these distributions into a quantitative assessment of the relative volume content of various species. This approach to fibril analysis facilitates rapid quantitative analysis of polymorphic species in heterogeneous samples of amyloid fibrils and other nano-structured materials.

Graphical abstract: Quantitative analysis of amyloid polymorphism using height histograms to correct for tip convolution effects in atomic force microscopy imaging

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Publication details

The article was received on 27 Sep 2016, accepted on 01 Dec 2016 and first published on 02 Dec 2016


Article type: Paper
DOI: 10.1039/C6RA24031C
RSC Adv., 2016,6, 114286-114295

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    Quantitative analysis of amyloid polymorphism using height histograms to correct for tip convolution effects in atomic force microscopy imaging

    Y. Lin, H. Komatsu, J. Ma, P. H. Axelsen and Z. Fakhraai, RSC Adv., 2016, 6, 114286
    DOI: 10.1039/C6RA24031C

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