Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 116, 2016
Previous Article Next Article

Protein thermostability engineering

Author affiliations


The use of enzymes for industrial and biomedical applications is limited to their function at elevated temperatures. The principles of thermostability engineering need to be implemented for proteins with low thermal stability to broaden their applications. Therefore, understanding the thermal stability modulating factors of proteins is necessary for engineering their thermostability. In this review, first different thermostability enhancing strategies in both the sequence and structure levels, discovered by studying the natural proteins adapted to different conditions, are introduced. Next, the progress in the development of various computational methods to engineer thermostability of proteins by learning from nature and introducing several popular tools and algorithms for protein thermostability engineering is highlighted. Further discussion includes the challenges in the field of protein thermostability engineering such as the protein stability–activity trade-off. Finally, how thermostability engineering could be instrumental for the design of protein drugs for biomedical applications is demonstrated.

Graphical abstract: Protein thermostability engineering

Back to tab navigation

Publication details

The article was received on 01 Jul 2016, accepted on 07 Nov 2016 and first published on 07 Nov 2016

Article type: Review Article
DOI: 10.1039/C6RA16992A
Citation: RSC Adv., 2016,6, 115252-115270

  •   Request permissions

    Protein thermostability engineering

    H. P. Modarres, M. R. Mofrad and A. Sanati-Nezhad, RSC Adv., 2016, 6, 115252
    DOI: 10.1039/C6RA16992A

Search articles by author