Jump to main content
Jump to site search

Issue 75, 2016
Previous Article Next Article

Fluorescent tracer of dopamine enables selective labelling and interrogation of dopaminergic amacrine cells in the retina of living zebrafish

Author affiliations

Abstract

The retinal dopaminergic amacrine cell plays multiple roles in vision; it optimizes the encoding of visual stimuli, serves as an output of the circadian rhythm, and influences trophic processes. Specific identification of such cells in vivo remains challenging. A fluorescent tracer that mimics natural dopamine would help elucidate the distribution and the functional roles of dopaminergic neurons. Here we report a fluorescent tracer of dopamine for the selective labeling and study of retinal dopaminergic amacrine cells in vivo. This tracer has low toxicity and effective selectivity, and enables fluorescent determination of the evoked release of dopamine and the femtosecond laser ablation of single dopaminergic amacrine cells in living zebrafish. Our approach is readily adaptable by other research groups, and is expected to accelerate biomedical research in domains related to the pathophysiology of retinal dopaminergic amacrine cells.

Graphical abstract: Fluorescent tracer of dopamine enables selective labelling and interrogation of dopaminergic amacrine cells in the retina of living zebrafish

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 May 2016, accepted on 06 Jul 2016 and first published on 20 Jul 2016


Article type: Paper
DOI: 10.1039/C6RA13073A
RSC Adv., 2016,6, 71589-71595

  •   Request permissions

    Fluorescent tracer of dopamine enables selective labelling and interrogation of dopaminergic amacrine cells in the retina of living zebrafish

    H. Lin, Z. Hong, Y. Li and I. Liau, RSC Adv., 2016, 6, 71589
    DOI: 10.1039/C6RA13073A

Search articles by author

Spotlight

Advertisements