A novel bio-microcircuit for bio-assays

Abstract

In this study, a novel micro-system made of a fluid circuit board and various functional components, inspired by integrated microcircuit techniques, was developed. The circuit board was fabricated on a thermoplastic sheet using two-dimensional (2D) cutting technology. The functional components, including mixers, dilutors, reactors, pumps, retreaters, and detectors, were fabricated via paper (or membrane) cutting/folding and integrated into the system, similar to the way that electronic components are integrated into a micro-integrated circuit board. This system, validated through the rapid detection of human immunodeficiency virus (HIV) and the starch catabolism process, could potentially be another microfluidic system for analytical science.