Crosslinking catalysis-active center of hemin on the protein scaffold toward peroxidase mimic with powerful catalysis

Minmin Dong; Liyan Zhang; Rui Li; Shuying Li; Yao Jiang; Yuchun Qiao; Zhiqiang Duan; Ru Li; Quanfu Wang; Hua Wang

doi:10.1039/C6RA07139B

Crosslinking catalysis-active center of hemin on the protein scaffold toward peroxidase mimic with powerful catalysis†

Minmin Dong,^a Liyan Zhang,^a Rui Li,^a Shuying Li,^a Yao Jiang,^a Yuchun Qiao,^a Zhiqiang Duan,^a Ru Li,^a Quanfu Wang^b and Hua Wang*^a

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^a Shandong Province Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu City, Shandong Province 273165, P. R. China
E-mail: huawangqfnu@126.com
Web: http://wang.qfnu.edu.cn
Fax: +86 5374456306
Tel: +86 5374456306

^b School of Marine and Technology, Harbin Institute of Technology, Weihai, Shandong Province, P. R. China

Abstract

An enzyme mimic synthesis protocol has been proposed by simply cross-linking the redox active center of peroxidase onto a protein scaffold. Colorimetric assays and kinetic studies indicate that the developed peroxidase mimic can present much stronger catalysis and better aqueous stability than native hemin.

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Crosslinking catalysis-active center of hemin on the protein scaffold toward peroxidase mimic with powerful catalysis†

Abstract

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Crosslinking catalysis-active center of hemin on the protein scaffold toward peroxidase mimic with powerful catalysis

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