A real time colorimetric ‘two in one’ kit for tracking ppb levels of uric acid and Hg2+ in live HeLa S3 cells and Hg2+ induced keto–enol tautomerism

Chanda Kumari; Dibyendu Sain; Ashish Kumar; Sushanta Debnath; Partha Saha; Swapan Dey

doi:10.1039/C6RA06987H

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A real time colorimetric ‘two in one’ kit for tracking ppb levels of uric acid and Hg²⁺ in live HeLa S3 cells and Hg²⁺ induced keto–enol tautomerism†

Chanda Kumari,^a Dibyendu Sain,^a Ashish Kumar,^a Sushanta Debnath,^b Partha Saha^b and Swapan Dey*^a

Author affiliations

* Corresponding authors

^a Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand, India
E-mail: deyswapan77@yahoo.com

^b Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India

Abstract

A TBET based ‘turn-on’ fluorescent reagent with high Stokes shifts, exhibits selective and colorimetric detection of biologically important uric acid as well as toxic Hg²⁺. A new Hg²⁺ induced keto–enol tautomerism phenomenon associated with a solvatochromic effect was reported. The sensor has proved its real time application as an accurate ‘two in one’ kit for tracking uric acid and Hg²⁺ in live HeLa cells via a fluorescence bio-imaging technique.

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Article information

DOI: https://doi.org/10.1039/C6RA06987H
Article type: Paper
Submitted: 16 Mar 2016
Accepted: 19 Jun 2016
First published: 01 Jul 2016

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RSC Adv., 2016,6, 62990-62998

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A real time colorimetric ‘two in one’ kit for tracking ppb levels of uric acid and Hg²⁺ in live HeLa S3 cells and Hg²⁺ induced keto–enol tautomerism

C. Kumari, D. Sain, A. Kumar, S. Debnath, P. Saha and S. Dey, RSC Adv., 2016, 6, 62990 DOI: 10.1039/C6RA06987H

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