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Issue 66, 2016, Issue in Progress
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Stable over-expression of the human malate–aspartate NADH shuttle member Aralar I in PK15 cells improves energy metabolism and enhances proliferation of porcine circovirus-2

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Abstract

The considerable losses sustained by the pig industry due to porcine circovirus-2 (PCV2) could be avoided by using an attenuated vaccine. However, production of an attenuated PCV2 vaccine has not been satisfactory. Recently, we over-expressed the human malate–aspartate NADH shuttle member Aralar I in PK15 cells to enhance proliferation of PCV2. Compared with control PK15 cells, lactate accumulation in Aralar I over-expressing cells decreased by 44.8% (p < 0.01), whereas the mitochondrial NADH and pyruvate concentrations increased by 50% (p < 0.05) and 35.4% (p < 0.05), respectively. The ATP/ADP ratio and O2 uptake rate also increased by 39% (p < 0.05) and 37.8% (p < 0.01), respectively. Furthermore, the glutamine consumption rate increased by 36.4% (p < 0.01). Finally, compared with the PCV2 yield of infected control PK15 cells, the PCV2 yield of infected Aralar I over-expression cells was enhanced by 42.5% (p < 0.05). These results revealed that over-expression of Aralar I can improve the energy metabolism of PK15 cells and also enhance the replication of the PCV2 virus in PK15 cells. This study presents a method to improve energy metabolism and provides a valuable thinking to enhance virus vaccine production.

Graphical abstract: Stable over-expression of the human malate–aspartate NADH shuttle member Aralar I in PK15 cells improves energy metabolism and enhances proliferation of porcine circovirus-2

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Article information


Submitted
09 Mar 2016
Accepted
23 May 2016
First published
13 Jun 2016

RSC Adv., 2016,6, 61268-61277
Article type
Paper

Stable over-expression of the human malate–aspartate NADH shuttle member Aralar I in PK15 cells improves energy metabolism and enhances proliferation of porcine circovirus-2

Z. Xia, X. Yi and Y. Zhuang, RSC Adv., 2016, 6, 61268
DOI: 10.1039/C6RA06343H

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