Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 42, 2016, Issue in Progress
Previous Article Next Article

Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes

Author affiliations

Abstract

The effect of increasing molecular mobility, on hydrated polyrotaxane (PRX)-coated surfaces, on differentiation of mouse induced pluripotent stem cells (iPS cells) into cardiomyocytes was examined. PRX is composed of α-cyclodextrin (α-CD) threaded on linear poly(ethylene glycol) (PEG)-capped terminals with bulky end-groups. The degree of molecular mobility at the hydrated state (Mf) on the PRX surfaces can be varied by changing the number of threaded α-CDs. Rac1 expression was significantly upregulated for adhering iPS cells on the PRX surface with high Mf value, while it was downregulated on surfaces with low Mf value. Furthermore, the expression of N-cadherin, which is an important marker protein for cardiomyogenic differentiation of stem cells, was greatly upregulated for adhering iPS cells on the PRX surface with high Mf value, while those on surfaces with low Mf value showed low N-cadherin expression. Finally, the PRX surface with higher Mf value was found to be higher in cardiomyogenesis and beating colony formation from iPS cells, the extent of which was much higher than that on gelatin-coated surfaces. This suggests that surface hydrated molecular mobility, varied by varying a supramolecular PRX architecture on materials, plays a significant role in controlling cytoskeletal signaling pathways, eventually contributing to the direction of stem cell commitment.

Graphical abstract: Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes

Back to tab navigation

Publication details

The article was received on 13 Feb 2016, accepted on 28 Mar 2016 and first published on 29 Mar 2016


Article type: Paper
DOI: 10.1039/C6RA03967G
Citation: RSC Adv., 2016,6, 35668-35676

  •   Request permissions

    Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes

    J. Seo, M. Hirata, S. Kakinoki, T. Yamaoka and N. Yui, RSC Adv., 2016, 6, 35668
    DOI: 10.1039/C6RA03967G

Search articles by author

Spotlight

Advertisements