Issue 44, 2016

Study on the cross-linking effect of a natural derived oxidized chitosan oligosaccharide on the porcine acellular dermal matrix

Abstract

The purpose of this study is to investigate the cross-linking interaction between a natural derived oxidized chitosan oligosaccharide (OCOS) and the porcine acellular dermal matrix (pADM), and further evaluate the varying properties of the pADM after cross-linked. The shrinkage temperature (Ts) and cross-link density study indicate that the amino groups of lysine or hydroxylysine side groups of collagen could predominantly react with the available aldehyde group of OCOS to form a more stable Schiff's base leading to the improved hydrothermal stability of pADM. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and atomic force microscopy (AFM) analysis indicate that the structural integrity of collagen (i.e. D-periodicity structure and triple helix) is still maintained after the OCOS treatment. Meanwhile, the mechanical properties, hydrophilicity, collagenase degradation and cytotoxicity of pADM after cross-linking have been all promoted obviously. Above all, the cytocompatibility analysis implies that when the dosage of OCOS is less than 8%, introducing OCOS into pADM might be favorable for cell adhesion, growth and proliferation. Taken as a whole, the present study demonstrates that OCOS could serve as an ideal cross-linker for the chemical fixation of pADM, which are compatible with its potential applications in biomaterials.

Graphical abstract: Study on the cross-linking effect of a natural derived oxidized chitosan oligosaccharide on the porcine acellular dermal matrix

Article information

Article type
Paper
Submitted
05 Feb 2016
Accepted
24 Mar 2016
First published
24 Mar 2016

RSC Adv., 2016,6, 38052-38063

Study on the cross-linking effect of a natural derived oxidized chitosan oligosaccharide on the porcine acellular dermal matrix

Y. Chen, N. Dan, L. Wang, X. Liu and W. Dan, RSC Adv., 2016, 6, 38052 DOI: 10.1039/C6RA03434A

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