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Issue 46, 2016, Issue in Progress
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Platinum nanopetal-based potassium sensors for acute cell death monitoring

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Growing interest in the role of ions as cell death regulators has led to the consideration of K+, which plays a major role in events such as necrosis, apoptosis and osmotic shock. These mechanisms induce effluxes of K+, which can be measured to monitor such cellular events. In this work, we present a fast and simple template-free electrodeposition method for modifying electrodes on microfabricated Si-based platforms with Pt nanopetals. K+-selective electrodes were constructed by coupling such newly obtained Pt nanopetals, which were used as a solid contact, with PVC (polyvinyl chloride) K+-selective membranes. The drift over time was reduced by three orders of magnitude from several mV h−1 for bare electrodes to tens of μV h−1 when Pt nanopetals were used as an intermediate layer between the electrode and the selective membrane. The improved potential stability is comparable to the best values obtained by using solid-contact ion-selective electrodes based on other nanomaterials. The sensors exhibited near-Nernstian behavior and high selectivity for K+. By studying cell viability in relation to K+ measurements, we established a new correlation between the level of ions and the cell viability upon exposure to both osmotic shock and treatment with acetaminophen. The present method for the continuous and non-invasive monitoring of cell death in a bioreactor has potential applications in various biomedical domains.

Graphical abstract: Platinum nanopetal-based potassium sensors for acute cell death monitoring

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The article was received on 19 Jan 2016, accepted on 06 Apr 2016 and first published on 07 Apr 2016

Article type: Paper
DOI: 10.1039/C6RA01664B
RSC Adv., 2016,6, 40517-40526

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    Platinum nanopetal-based potassium sensors for acute cell death monitoring

    I. Taurino, S. Massa, G. Sanzó, J. Aleman, B. Flavia, S. R. Shin, Y. S. Zhang, M. R. Dokmeci, G. De Micheli, S. Carrara and A. Khademhosseini, RSC Adv., 2016, 6, 40517
    DOI: 10.1039/C6RA01664B

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