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Issue 33, 2016
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Introduction of an α,β-unsaturated carbonyl conjugated pyrene–lactose hybrid as a fluorescent molecular probe for micro-scale anisotropic media

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Abstract

A new fluorescent lactose molecule (pyd-lact) (E)-1-(galactose-β-(1→4)-β-D-glucopyranosyl)-4-(1-pyrene)-but-3-en-2-one, has been synthesized by attaching 1-pyrene-but-3-ene-2-one to lactose. Extended π-conjugation removes the emission forbiddenness of pyrene, which results in the broad spectrum. Detailed photo-physical studies in homogeneous media show the sensitivity of ICT (intra molecular charge transfer) emission towards medium polarity. Unlike pyrene, pyd-lact is more soluble in water at a probe concentration of 10 μM, because of the attached disaccharide. Thus, the usage of pyd-lact as an aqueous probe is found to be advantageous over pyrene. Using the polarity sensitivity of fluorescence parameters (intensity, shift of emission maximum, anisotropy, lifetime), the micro-heterogeneity of anisotropic media such as β-CD, bile salts, and Pluronic P123 have been monitored. In contrast to pyrene-3-carboxaldehyde, which is often used as a hydrophobic fluorescent molecular probe, pyd-lact reports from the interfacial regions of micro-heterogeneous media.

Graphical abstract: Introduction of an α,β-unsaturated carbonyl conjugated pyrene–lactose hybrid as a fluorescent molecular probe for micro-scale anisotropic media

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Publication details

The article was received on 08 Dec 2015, accepted on 08 Mar 2016 and first published on 10 Mar 2016


Article type: Paper
DOI: 10.1039/C5RA26146E
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Citation: RSC Adv., 2016,6, 27933-27943
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    Introduction of an α,β-unsaturated carbonyl conjugated pyrene–lactose hybrid as a fluorescent molecular probe for micro-scale anisotropic media

    I. Sarkar, A. Hemamalini, T. M. Das and A. K. Mishra, RSC Adv., 2016, 6, 27933
    DOI: 10.1039/C5RA26146E

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