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Issue 14, 2016
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Photosynthesis of cyanobacteria in a miniaturized optofluidic waveguide platform

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Abstract

We investigated the effect of increasing the optical penetration length, inside polydimethylsiloxane (PDMS)-based photobioreactors (PBRs), upon the photosynthetic cell growth of cyanobacteria. A thin layer of Teflon amorphous fluoropolymer (Teflon AF) was applied inside the PDMS-based PBRs to prevent light loss at the solid–liquid interface. The Teflon AF layer, with a refractive index (nTeflon = 1.31) lower than PDMS (nPDMS = 1.442) and higher than the culture medium (nmedium = 1.332), constructed the light waveguide in the PBRs via the total internal reflection. Such a combination of refractive indices led to the prevention of light loss at the interface. The cell growth rate and the optical cell density were measured periodically for 5 days under different light power and Teflon AF-coating conditions. The local or global auto-fluorescence signal and the optical density at 450 nm wavelength (OD450) were measured in parallel by a fluorescence microscope and a micro plate reader, respectively. The optofluidic waveguide-based PBR improved the photosynthetic cell growth up to ∼9% compared to a regular PBR.

Graphical abstract: Photosynthesis of cyanobacteria in a miniaturized optofluidic waveguide platform

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Article information


Submitted
18 Nov 2015
Accepted
18 Jan 2016
First published
20 Jan 2016

RSC Adv., 2016,6, 11081-11087
Article type
Paper
Author version available

Photosynthesis of cyanobacteria in a miniaturized optofluidic waveguide platform

J. H. Jung, K. S. Lee, S. Im, G. Destgeer, B. H. Ha, J. Park and H. J. Sung, RSC Adv., 2016, 6, 11081
DOI: 10.1039/C5RA24344K

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