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Issue 12, 2016
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Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria

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Abstract

Here, we provide the first evidence of yeast strains assisted Ag/AgCl-NPs production in vitro. The formed nanoparticles were characterized by spectroscopic and electron microscopy approaches. UV-vis supported the biosynthesis. TEM analysis evidenced that the nanoparticles mainly presented a circular shape and their diameters varied mostly being in the range 2 to 10 nm. XRD analysis showed a crystalline structure, with diffraction peaks corresponding to metallic silver and silver chloride nanoparticles, and when analyzed by high-resolution transmission electron microscopy (HRTEM), instead of being round, (111) (octahedral) and (200) (cubic) symmetry facets appeared systematically in one side of the nanoparticles. Analysis of ultra-thin sections by TEM indicated that the domain of the synthesis of Ag/AgCl-NPs was mainly between the cell wall and the plasma membrane. By using 3D reconstruction obtained from focused ion beam scanning electron microscopy (FIB/SEM) the spatial distribution of the domains of nanoparticle synthesis was mapped and nanoaggregates of Ag/AgCl-NPs up 35 nm in diameter were observed. Extracellular synthesis also occurred; in accordance with the fact that conditioned media from yeast isolates were as efficient at producing Ag/AgCl-NPs as live-cell cultures. Exposure of Gram-positive Staphylococcus aureus and Gram-negative Klebsiella pneumoniae cultures to Ag/AgCl-NPs led to a strong growth inhibition as shown by optical density measurements. The Ag/AgCl-NPs described here have characteristics compatible with a strong potential for use in the biotechnology industry, particularly for biomedical applications.

Graphical abstract: Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria

  • This article is part of the themed collection: Rio 2016
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The article was received on 29 Oct 2015, accepted on 15 Jan 2016 and first published on 18 Jan 2016


Article type: Paper
DOI: 10.1039/C5RA22727E
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RSC Adv., 2016,6, 9893-9904

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    Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria

    M. Eugenio, N. Müller, S. Frasés, R. Almeida-Paes, L. M. T. R. Lima, L. Lemgruber, M. Farina, W. de Souza and C. Sant'Anna, RSC Adv., 2016, 6, 9893
    DOI: 10.1039/C5RA22727E

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