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Issue 22, 2016
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Design, synthesis and in vitro evaluation of anticancer and antibacterial potential of surface modified Tb(OH)3@SiO2 core–shell nanoparticles

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Abstract

In the current study, we modified the surface of Tb(OH)3 nanoparticles with a silica layer to enhance their solubility and biocompatibility. Transmission electron microscopy confirmed the improvements in these properties. Tb(OH)3@SiO2 core–shell nanoparticles (TS-CSNPs) exhibited a strong green emission peak upon irradiation with ultraviolet light, which originates from the electric-dipole transition 5D47F5 (543 nm) of the Tb3+ ion. In vitro anticancer and antimicrobial activities of the synthesized TS-CSNPs has been evaluated through their potential cytotoxicity and antibacterial activity. TS-CSNPs were shown to have more cytotoxicity against HT29 human colorectal cancer cells with a value of IC50 420.33 in an MTT assay. The alteration of the morphological features of HT29 cells was analysed using various concentrations of TS-CSNPs by inverted microscopy. Western blot analysis results of the apoptotic pathway showed that TS-CSNPs inhibited the growth of HT29 cancer cells through the induction of apoptosis, as evidenced by the down regulation of the expression of anti-apoptotic Bcl-2 and Bcl-xL gene products. Furthermore, the results of the in vitro hemolysis assay with human erythrocytes demonstrated the excellent blood biocompatibility of TS-CSNPs. Our silica coated TS-CSNPs exhibited a non-significant effect on the viability of both Gram negative and Gram positive bacterial strains up to 18 hours of exposure. These results highlight that modified TS-CSNPs can be functionalized to enhance the efficacy of cancer therapeutics due to the significant potential against cancerous cells and antibacterial activity.

Graphical abstract: Design, synthesis and in vitro evaluation of anticancer and antibacterial potential of surface modified Tb(OH)3@SiO2 core–shell nanoparticles

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Article information


Submitted
03 Sep 2015
Accepted
25 Jan 2016
First published
27 Jan 2016

RSC Adv., 2016,6, 18667-18677
Article type
Paper

Design, synthesis and in vitro evaluation of anticancer and antibacterial potential of surface modified Tb(OH)3@SiO2 core–shell nanoparticles

S. Khan, A. A. Ansari, A. A. Khan, W. Al-Kattan, O. Al-Obeed and R. Ahmad, RSC Adv., 2016, 6, 18667
DOI: 10.1039/C5RA17906H

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