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Issue 2, 2016
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Quenching the firefly bioluminescence by various ions

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Abstract

The luciferase reporter gene assay system is broadly applied in various biomedical aspects, including signaling pathway dissection, transcriptional activity analysis, and genetic toxicity testing. It significantly improves the experimental accuracy and reduces the experimental error by the addition of an internal control. In the current research, we discovered some specific ions that could selectively inhibit firefly luciferase while having a negligible effect on renilla luciferase in vitro in the dual-reporter gene assay. We showed that these ionic compounds had a high potential of being utilized as quench-and-activate reagents in the dual-reporter assay. Furthermore, results from kinetic studies on ion-mediated quenching effects indicated that different ions have distinct inhibition modes. Our study is anticipated to guide a more affordable design of quench-and-activate reagents in biomedicine and pharmaceutical analysis.

Graphical abstract: Quenching the firefly bioluminescence by various ions

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Supplementary files

Article information


Submitted
25 Nov 2015
Accepted
05 Jan 2016
First published
07 Jan 2016

Photochem. Photobiol. Sci., 2016,15, 244-249
Article type
Paper
Author version available

Quenching the firefly bioluminescence by various ions

H. Zhang, H. Bai, T. Jiang, Z. Ma, Y. Cheng, Y. Zhou, L. Du and M. Li, Photochem. Photobiol. Sci., 2016, 15, 244
DOI: 10.1039/C5PP00432B

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