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Issue 39, 2016
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Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

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Abstract

A transaminase from Halomonas elongata and four mutants generated by an in silico-based design were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives were excellent substrates, only NO2-acetophenones were transformed into the (S)-amine with a high enantioselectivity. The different behaviour of wild-type and mutated transaminases was assessed by in silico substrate binding mode studies.

Graphical abstract: Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

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Publication details

The article was received on 29 Jul 2016, accepted on 06 Sep 2016 and first published on 06 Sep 2016


Article type: Paper
DOI: 10.1039/C6OB01629D
Citation: Org. Biomol. Chem., 2016,14, 9306-9311

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    Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

    M. L. Contente, M. Planchestainer, F. Molinari and F. Paradisi, Org. Biomol. Chem., 2016, 14, 9306
    DOI: 10.1039/C6OB01629D

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