Issue 39, 2016

5-Bromo-2′-deoxycytidine—a potential DNA photosensitizer


A double-stranded oligonucleotide, 80 base pairs in length, was multiply labeled with 5-bromo-2′-deoxycytidine (BrdC) using polymerase chain reaction (PCR). The modified oligonucleotide was irradiated with 300 nm photons and its damage was assayed by employing DHPLC, LC-MS and denaturing polyacrylamide gel electrophoresis (PAGE). Two types of damage were demonstrated, namely, single strand breaks (SSBs) and intrastrand cross-links (ICLs); the ICLs were in the form of d(G^C) and d(C^C) dimers. The former species are probably formed due to photoinduced electron transfer between the photoexcited BrdC and the ground state 2′-deoxyguanosine (dG), whereas the latter is a result of a cycloaddition reaction. Since SSBs and ICLs are potentially lethal to the cell, BrdC could be considered as a nucleoside with possible clinical applications.

Graphical abstract: 5-Bromo-2′-deoxycytidine—a potential DNA photosensitizer

Supplementary files

Article information

Article type
06 Jul 2016
30 Aug 2016
First published
30 Aug 2016
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2016,14, 9312-9321

5-Bromo-2′-deoxycytidine—a potential DNA photosensitizer

M. Zdrowowicz, P. Wityk, B. Michalska and J. Rak, Org. Biomol. Chem., 2016, 14, 9312 DOI: 10.1039/C6OB01446A

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