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Issue 24, 2016
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Site-directed spin labeling of proteins for distance measurements in vitro and in cells

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Abstract

Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.

Graphical abstract: Site-directed spin labeling of proteins for distance measurements in vitro and in cells

  • This article is part of the themed collection: New Talent
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Publication details

The article was received on 01 Mar 2016, accepted on 05 May 2016 and first published on 05 May 2016


Article type: Review Article
DOI: 10.1039/C6OB00473C
Citation: Org. Biomol. Chem., 2016,14, 5468-5476
  • Open access: Creative Commons BY license
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    Site-directed spin labeling of proteins for distance measurements in vitro and in cells

    P. Roser, M. J. Schmidt, M. Drescher and D. Summerer, Org. Biomol. Chem., 2016, 14, 5468
    DOI: 10.1039/C6OB00473C

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