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Abstract | Cited by

PS B, a naturally occurring CD4+ T-cell simulating zwitterionic polysaccharide from Bacteroides fragilis ATCC 25285/NCTC 9343, was conjugated with aminooxy Thomsen Friedenreich (TF or T) [α-D-Gal-(1,3)-β-D-GalNAc-ONH2] tumor antigen. Immunization in Jax C57BL/6, followed by ELISA revealed IgM and IgG antibody TF specificity. FACS data noted preferential binding to TF-laced MCF-7 cells but not to HCT-116 cells.

Graphical abstract: Immunological evaluation of the entirely carbohydrate-based Thomsen-Friedenreich – PS B conjugate

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