Ming-Qi Wang, Juan Dong, Huafan Zhang and Zhuo Tang
Org. Biomol. Chem., 2016,14, 2347-2351
DOI:
10.1039/C6OB00148C,
Paper
We identified a new class of deoxyribozymes named A-2 and A-3 by in vitro selection which required both Cu2+ and Mn2+ as cofactors that selectively and rapidly cleave the DNA substrate. Studies confirmed that they cleaved via a mechanistic pathway involving the formation of hydrogen peroxide as the reactive species. The kinetics, secondary structures and sequence tolerance of the new class of the deoxyribozymes A-2 and A-3 were reported.