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Issue 30, 2016
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Nanoparticle mechanics: deformation detection via nanopore resistive pulse sensing

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Abstract

Solid-state nanopores have been widely used in the past for single-particle analysis of nanoparticles, liposomes, exosomes and viruses. The shape of soft particles, particularly liposomes with a bilayer membrane, can greatly differ inside the nanopore compared to bulk solution as the electric field inside the nanopores can cause liposome electrodeformation. Such deformations can compromise size measurement and characterization of particles, but are often neglected in nanopore resistive pulse sensing. In this paper, we investigated the deformation of various liposomes inside nanopores. We observed a significant difference in resistive pulse characteristics between soft liposomes and rigid polystyrene nanoparticles especially at higher applied voltages. We used theoretical simulations to demonstrate that the difference can be explained by shape deformation of liposomes as they translocate through the nanopores. Comparing our results with the findings from electrodeformation experiments, we demonstrated that the rigidity of liposomes can be qualitatively compared using resistive pulse characteristics. This application of nanopores can provide new opportunities to study the mechanics at the nanoscale, to investigate properties of great value in fundamental biophysics and cellular mechanobiology, such as virus deformability and fusogenicity, and in applied sciences for designing novel drug/gene delivery systems.

Graphical abstract: Nanoparticle mechanics: deformation detection via nanopore resistive pulse sensing

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Supplementary files

Article information


Submitted
25 Apr 2016
Accepted
03 Jun 2016
First published
06 Jun 2016

Nanoscale, 2016,8, 14420-14431
Article type
Paper
Author version available

Nanoparticle mechanics: deformation detection via nanopore resistive pulse sensing

A. Darvish, G. Goyal, R. Aneja, R. V. K. Sundaram, K. Lee, C. W. Ahn, K. Kim, P. M. Vlahovska and M. J. Kim, Nanoscale, 2016, 8, 14420
DOI: 10.1039/C6NR03371G

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